Friday, June 4, 2021

How to Boost Muscle Regeneration and Rebuild Tissue

One of the many effects of aging is loss of muscle mass, which contributes to disability in older people. To counter this loss, scientists at the Salk Institute are studying ways to accelerate the regeneration of muscle tissue, using a combination of molecular compounds that are commonly used in stem-cell research.

In a study published on May 25, 2021, in Nature Communications, the investigators showed that using these compounds increased the regeneration of muscle cells in mice by activating the precursors of muscle cells, called myogenic progenitors. Although more work is needed before this approach can be applied in humans, the research provides insight into the underlying mechanisms related to muscle regeneration and growth and could one day help athletes as well as aging adults regenerate tissue more effectively.

“Loss of these progenitors has been connected to age-related muscle degeneration,” says Salk Professor Juan Carlos Izpisua Belmonte, the paper’s senior author. “Our study uncovers specific factors that are able to accelerate muscle regeneration, as well as revealing the mechanism by which this occurred.”

Induction of Yamanaka factors (OKSM) in muscle fibers increases the number of myogenic progenitors. Top, control; bottom, treatment. Red-pink color is Pax7, a muscle stem-cell marker. Blue indicates muscle nuclei.
Credit: Salk Institute

The compounds used in the study are often called Yamanaka factors after the Japanese scientist who discovered them. Yamanaka factors are a combination of proteins (called transcription factors) that control how DNA is copied for translation into other proteins. In lab research, they are used to convert specialized cells, like skin cells, into more stem-cell-like cells that are pluripotent, which means they have the ability to become many different types of cells.

“Our laboratory previously showed that these factors can rejuvenate cells and promote tissue regeneration in live animals,” says first author Chao Wang, a postdoctoral fellow in the Izpisua Belmonte lab. “But how this happens was not previously known.”

Muscle regeneration is mediated by muscle stem cells, also called satellite cells. Satellite cells are located in a niche between a layer of connective tissue (basal lamina) and muscle fibers (myofibers). In this study, the team used two different mouse models to pinpoint the muscle stem-cell-specific or niche-specific changes following addition of Yamanaka factors. They focused on younger mice to study the effects of the factors independent of age.

In the myofiber-specific model, they found that adding the Yamanaka factors accelerated muscle regeneration in mice by reducing the levels of a protein called Wnt4 in the niche, which in turn activated the satellite cells. By contrast, in the satellite-cell-specific model, Yamanaka factors did not activate satellite cells and did not improve muscle regeneration, suggesting that Wnt4 plays a vital role in muscle regeneration.

According to Izpisua Belmonte, who holds the Roger Guillemin Chair, the observations from this study could eventually lead to new treatments by targeting Wnt4.

“Our laboratory has recently developed novel gene-editing technologies that could be used to accelerate muscle recovery after injury and improve muscle function,” he says. “We could potentially use this technology to either directly reduce Wnt4 levels in skeletal muscle or to block the communication between Wnt4 and muscle stem cells.”

The investigators are also studying other ways to rejuvenate cells, including using mRNA and genetic engineering. These techniques could eventually lead to new approaches to boost tissue and organ regeneration.

Other authors included: Ruben Rabadan Ros, Paloma Martinez Redondo, Zaijun Ma, Lei Shi, Yuan Xue, Isabel Guillen-Guillen, Ling Huang, Tomoaki Hishida, Hsin-Kai Liao, Concepcion Rodriguez Esteban, and Pradeep Reddy of Salk; Estrella Nuñez Delicado of Universidad Católica San Antonio de Murcia in Spain; and Pedro Guillen Garcia of Clinica CEMTRO in Spain.

The work was funded by NIH-NCI CCSG: P30 014195, the Helmsley Trust, Fundacion Ramon Areces, Asociación de Futbolistas Españoles (AFE), Fundacion Pedro Guillen, Universidad Católica San Antonio de Murcia (UCAM), the Moxie Foundation and CIRM (GC1R-06673-B).


Contacts and sources:
Salk Institute


PROTECT WITH IMMUSIST



Publication: In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche.

Chao Wang, Ruben Rabadan Ros, Paloma Martinez-Redondo, Zaijun Ma, Lei Shi, Yuan Xue, Isabel Guillen-Guillen, Ling Huang, Tomoaki Hishida, Hsin-Kai Liao, Estrella Nuñez Delicado, Concepcion Rodriguez Esteban, Pedro Guillen-Garcia, Pradeep Reddy, Juan Carlos Izpisua Belmonte. Nature Communications, 2021; 12 (1) DOI: 10.1038/s41467-021-23353-z

15 comments:

  1. Hello viewers around the Globe, I was despondent because i had a very small penis, about 2.5 inches soft and 4 inches hard not nice enough to satisfy a woman, i have been in so many relationship, but cut off because of my situation, i have used so many product which doctors for me, but none could offer me the help i searched for. i saw some few comments on the INTERNET about this specialist called Dr,OLU and decided to contact him on his email: Drolusolutinthome@gmail.com) so I decided to give his herbal product a try. i emailed him and he got back to me, he gave me some comforting words with his herbal pills for Penis Enlargement, Within 3 week of it, i began to feel the enlargement was surprised when she said that she is satisfied with my sex and i have got a large penis. Am so happy, thanks to Dr OLU I also learn that Dr OLU also help with Breast Enlargement Hips and Bums Enlargement etc.. If you are in any situation with a little Penis, weak ejaculation, small breast_hips_bums do get to Dr OLU now for help on his email ( Drolusolutionhome@gmail.com ) or add him on whatsapp line +2348140654426 

    ReplyDelete
  2. This is another testimony on how Chief Dr Lucky cured my HIV disease. Do you need a cure for your HIV disease? Do you want to be cured from your cancer disease? Or you want to be free from any type of disease. Kindly visit his website https://chiefdrluckyherbaltherapy.wordpress.com/ , he just cured my HIV disease and I’m very grateful to him, he is the only herbalist that can cure you.  
    WhatsApp number : +2348132777335 
    Via Email : chiefdrlucky@gmail.com
    Thank you all for reading,
    God bless"

    ReplyDelete
  3. Thank you for giving me a good understanding of tissue regeneration and muscle formation. Very detailed and easy to understand article! I will follow you!
    snowball io

    ReplyDelete
  4. Functional Medicine KL that brings together conventional medicine, nutritional medicine, and bio-identical hormone therapy to restore health and enhance youth. Our doctors are here to educate, inspire & empower you to make better decisions regarding your health.

    ReplyDelete

  5. Howdy! Someone in my Myspace group shared this website with us so
    I came to give it a look. I'm definitely loving the information. I'm bookmarking and will be tweeting this to my followers!
    Superb blog and superb design and style.

    Feel free to surf to my blog - 오피사이트

    ReplyDelete
  6. Hello, beautiful people! Try playing Slope unblocked go, will you?

    ReplyDelete
  7. I saw your article well. You seem to enjoy 카지노사이트 for some reason. We can help you enjoy more fun. Welcome anytime :-)


    ReplyDelete
  8. GVS Malaysia
    was founded with a goal to advance positively alongside the local community and in tandem with the group’s strategic expansion plan that would serve both foreign industries and locals alike.

    ReplyDelete
  9. This place inspires me, thanks for sharing it with us. babyperiod.com

    ReplyDelete
  10. basketball stars unblocked
    I'm glad I discovered this place. I will visit often.

    ReplyDelete
  11. Bone Meal started out with an incredible objective of ensuring environmental sustainability through innovative green engineering. Our journey began as a fertilizer manufacturing company, utilizing available wastes and converting them into value-added nutrition for plants.

    ReplyDelete
  12. Nitrogen fertilizer started out with an incredible objective of ensuring environmental sustainability through innovative green engineering. Our journey began as a fertilizer manufacturing company, utilizing available wastes and converting them into value-added nutrition for plants.

    ReplyDelete
  13. USDA certified organic input started out with an incredible objective of ensuring environmental sustainability through innovative green engineering. Our journey began as a fertilizer manufacturing company, utilizing available wastes and converting them into value-added nutrition for plants.

    ReplyDelete
  14. EU certified organic input started out with an incredible objective of ensuring environmental sustainability through innovative green engineering. Our journey began as a fertilizer manufacturing company, utilizing available wastes and converting them into value-added nutrition for plants.

    ReplyDelete