Sunday, April 29, 2012

Bioluminescent Technology For Easy Tracking Of GMO

It is important to be able to monitor genetically modified (GM) crops, not only in the field but also during the food processing chain. New research published in BioMed Central's open access journal BMC Biotechnology shows that products from genetically modified crops can be identified at low concentration, using bioluminescent real time reporter (BART) technology and loop mediated isothermal amplification (LAMP). The combination of these techniques was able to recognise 0.1% GM contamination of maize, far below the current EU limit of 0.9%.

File:Commercial growth of GM crops in 2011.jpg
Credit: Wikipedia

In agriculture GM crops have been bred to improve crop yield or viability. For example some are resistant to herbicides or viruses. They are also used in the pharmaceutical industry to produce proteins such as collagen. However there is a constant debate about the safety of these crops and whether the man-made transgenes might enter the wild population by cross-fertilization.and produce herbicide resistant weeds.

Careful handling and sampling techniques are required to assess the GM content of a crop. The most common technique is polymerase chain reaction (PCR), however, this involves complex extraction procedures and rapid thermocycling, both of which require specific equipment. To overcome these problems researchers from Lumora Ltd. assessed whether they could use LAMP to amplify DNA at a constant temperature and use BART to identify GM-specific DNA in real time.

Dr Guy Kiddle from Lumora, who led the research, explained that LAMP-BART was able to detect as little as 0.1% GM contamination of maize, and, compared to PCR, was more tolerant of contaminating polysaccharides, meaning that the DNA clean-up process did not need to be as thorough. He commented, "This method requires only basic equipment for DNA extraction, and a constant temperature for DNA amplification and detection. Consequently LAMP-BART provides a 'field-ready' solution for monitoring GM crops and their interaction with wild plants or non-GM crops."



Contacts and sources:
Dr Hilary Glover
BioMed Central

Citation:   GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use Guy Kiddle, Patrick Hardinge, Neil Buttigieg, Olga Gandelman, Clint Pereira, Cathal J McElgunn, Manuela Rizzoli, Rebecca Jackson, Nigel Appleton, Cathy Moore, Laurence C. Tisi and James A.H. Murray BMC Biotechnology (in press)

BioMed Central (http://www.biomedcentral.com/) is an STM (Science, Technology and Medicine) publisher which has pioneered the open access publishing model. All peer-reviewed research articles published by BioMed Central are made immediately and freely accessible online, and are licensed to allow redistribution and reuse. BioMed Central is part of Springer Science+Business Media, a leading global publisher in the STM sector.

2 comments:

  1. Hi,
    While browsing I went through your blog and found it something different and I was paused to think for a while that seriously what would be the life of the people without technology? Then by just saying to myself “No…Life is not possible”, I just move on. People are so acquainted with all the modern technologies that it is very difficult to think of the life without it. Looking for a post regarding whether this improve technology is useful or harmful?
    Orimark

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  2. "It is important to be able to monitor genetically modified (GM) crops, not only in the field but also during the food processing chain."

    -Yes, and i think they already passed a bill about GMO labeling. I'm in favor in it, labeling both GMO and organic is much better.

    ReplyDelete