Researchers at College of Science of University of Tehran in tight collaboration with their colleagues at Stem Cell Technology Research Center, Iran, exploited nanofibrous poly (lactic acid) scaffolds coated with hydroxyapatite nanoparticles for bone tissue engineering applications.
"In the conducted research, nanofibrous poly (lactic acid) scaffolds were synthesized via electrospinning in the first phase. The mentioned scaffolds underwent plasma processing for superficial modifications. Prior to that, nanohydroxyapatite (n-HA) was coated on the scaffolds," Ehsan Seyejafari, the chief researcher of the study, said to the news service of INIC.
According to Seyejafari, the synthesis procedure of the discussed scaffolds is so simple and cost-effective that their production on an industrial scale looks very likely. Coating nanohydroxyapatite particles upon the nanofibers provides direct contact onto cells and surrounding tissues. As a result, their bioactivity effect for osteogenic repair and regeneration is enhanced.
"Electron microscopic analyses revealed that all the scaffolds possessed nanofibrous structures of planar type and the superficial modifications brought about no changes on their development," Seyejafari reiterated, elaborating on the procedure of the research.
"Furthermore, nanoparticles were perfectly coated upon nanofibers' surfaces and did not disturb scaffold porosity. FTIR ascertained the presence of nanohydroxyapatite particles attached to nanofibers by the relevant peaks. Nanofibers' surfaces became highly hydrophilic and their contact angle inclined towards zero."
"Cell growth and multiplication tests confirmed biocompatibility of the nanostructures. In vitro investigations in mice demonstrated that the fabricated scaffolds are capable of simulating osteogenesis and forming the bony baffles," he concluded.
An elaborated report on the researchers' findings has been published in the Journal of Biomacromolecules, vol. 11, pages 3118-3125, 2010.